Frequently asked questions
What is Ongo Sperm Test?
Ongo sperm test is a device for the assessment of semen motility and concentration. It is calibrated for use with the specified species only. It is suitable for users who are involved in semen collection, preparation and processing, or artificial insemination.
Ongo sperm test is designed and calibrated for use in combination with Ongo slides and should only be used in this way.
How do I make measurement? How does it work?
Place an Ongo slide onto the stage. Choose the menu item “start”. The stage will begin warming up. When the displayed temperature reaches 37°C, a 5 µl sample can be pipetted into the right-hand chamber.
Start by pressing the “-“ button. Use the toggle button and adjust the lighting to obtain optimal contrast. Choose a field of view without artifacts. When selecting a field of view, make sure the left side of the slide is pushed up against the edge to ensure correct placement of the chamber. Press “record”. A two second video is recorded at 30 fps and analyzed, and the results are displayed.
What are the results?
The results screen indicates the sample ID and lists values for cell count, concentration (million sperm/ml), progressive motility (%), total motility (%), and immotile cells (%).
How do I make repeated measurements on the same slide?
Measurements of the same sample can be continued with a further video by pressing the “repeat” button, or results can be saved and the measurement process of the current sample concluded by pressing the “finish” button.
I got "drift" error message. What does it mean?
If movement of all objects in one direction is detected, the error message “drift” will be displayed on the results screen. In this case, make sure that drift from filling the chamber has subsided. Wait a few seconds before recording the first video there is no excess semen after filling the chamber. 5 µl of the sample should be used the device is placed on a level surface and undisturbed during measurements.
What does "low cell count" error meassage mean?
Low cell count: If fewer than 20 cells are measured in one video, the error message “low cell count” will be displayed on the results screen. In this case, make sure that the Ongo slide is placed correctly (see above), the chamber is completely filled (5µl), the sample is not too dilute.
Ongo bounces back from "Heating ... Please wait".
In case of low battery power, Ongo will return to the main screen. If a measurement process is underway, it will not be aborted, however the stage will no longer be heated.
Ongo keeps displaying “Put Ongo slide with sample".
Ongo slides are authenticated by the device. In case of authentication failure, the prompt “Put Ongo slide with sample” will be displayed. In case of authentication failure of an Ongo slide, please put a new Ongo slide with sample and send the failed Ongo slide to us.
What data are saved to pendrive?
All data can be found on the pen drive in a folder corresponding to the sample ID on the results screen. The files stored in each folder are:
- a PDF file listing the results of the individual videos as well as the averaged results
- MP4 videos of each measurement
What kind of care does Ongo need?
Protect Ongo sperm test from water and temperatures outside 0-50°C. Always use transport case and padding when transporting or not in use. Clean with a dry cloth. Clean the lens cover with a non-scratching textile suitable for optical equipment. To retain warranty, only use the power supply provided in the Ongo Starter Kit. Warranty is void if the device is opened or modified.
What do I need to watch out?